By Kim Gail Clarke
With this publication, the writer goals to generate middle abilities via an realizing of the complementary biotechnology disciplines and their interdependence. Chapters advance kinetic and mass move ideas that quantify optimal technique functionality and scale up. The textual content is large in scope, in terms of bioprocesses utilizing bacterial, fungal and enzymic biocatalysts, resting and energetic cells, batch, fed-batch and non-stop concepts and loose and immobilized configurations.
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Extra info for Bioprocess engineering: An introductory engineering and life science approach
Biochemical changes take place in a series of intermediate steps, each catalysed by a specific enzyme, and are referred to as the metabolic pathways or the metabolism of the microorganism. In the bioprocess, however, the needs of the microorganism to live and reproduce are replaced by the drive to enhance product formation. Process conditions are manipulated so that the microorganism is exploited to overproduce the desired product and maximising the production potential of the microorganism becomes the core focus.
G. pH). For instance, Penicilliun canescens and Aspergillus niger exist as a filamentous mass and pellets respectively during production of glucose oxidase, an enzyme which serves as a diagnostic tool in blood glucose determination and as an additive to prevent rancidity in foods. While filamentous morphology is known to have the potential to influence the production kinetics indirectly through its alteration of the fluid properties, it may also have a direct impact on product accumulation. When considering the walls of hyphae, the lateral walls have been shown to be 14 Published by Woodhead Publishing Limited, 2013 Microbiology rigid with pores that are too small to allow excretion of large molecules such as enzymes.
The prokaryotic bacteria are frequently able to sporulate where endospores, and later mature spores, develop under adverse environmental conditions. These non-vegetative forms are capable of germinating after exceedingly long periods (up to 20 years in soil) and are resistant to stress, especially high temperatures. In fact, short periods of high 11 Published by Woodhead Publishing Limited, 2013 Bioprocess engineering temperatures can actually stimulate spores into vegetative growth. g. Bacillus stearothermophilus, form spores which are particularly resistant to temperature.
Bioprocess engineering: An introductory engineering and life science approach by Kim Gail Clarke